Fluorescence in situ hybridization (FISH) is a powerful method to directly visualise the localisation of genomic alterations in the nucleus. The technique has been adapted to a wide range of applications in both medicine, especially diagnostic cytogenetics, and biology. Topics described in this manual include:
FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, e.g. formalin-fixed and paraffin-embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and expression of apoptosis-related genes; comparative genomic hybridization; special FISH techniques (Fiber-, PNA-, CO-, NU- and DBD-FISH); FISH on insect cells and yeast; multi-color FISH applications (spectral karyotyping analysis, reverse FISH and FICTION); FISH-related techniques, such as PRINS and microarrays.
| Title: | Fish Technology | Publisher: | Springer |
| Author: | B W Rautenstraub, T Liehr, Bernd W Rautenstrau |
| Edition: | Paperback |
| Language: | English |
| ISBN: | 3540672761 |
| EAN: | 9783540672760 |
| No. of Pages: | 494 |
| Publish Date: | 2002-3-22 |
| Binding: | Paperback |
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