About the Book :
This book offers step-by-step practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering. It offers a unique focus on tissue engineering methods for cell sourcing and utilization, combining theoretical overviews and detailed procedures. It is divided into two parts. The first discusses in vitro cultivation procedures, and the second part covers a variety of tissue engineering applications. Written by leading experts in the field, each chapter gives a brief overview of basic principles, followed by detailed cell culture and tissue engineering procedures that incorporate state-of-the-art examples and case studies. Each chapter includes stepwise protocols for representative well established methods.
Interesting Facts :
Easy to use format with a two-part organization.Logically organized, part one discussing cell sourcing, preparation and characterization and the second part looking at specific engineered tissues.Integrates theoretical background and detailed step-by-step protocols for each system or application.Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studiesEnd-of-chapter features include useful listings of sources for reagents, materials, and supplies
Preface.List of Abbreviations.PART I: CELL CULTURE.1.Basic Principles of Cell Culture (R. Freshney).2.Mesenchymal Stem Cells for Tissue Engineering (D. Lennon & A. Caplan).3.Human Embryonic Stem cell Culture for Tissue Engineering (S. Levenberg, et al.).4.Cell Sources for Cartilage Tissue Engineering (B. Johnstone, et al.).5.Lipid-Mediated Gene Transfer for Cartilage Tissue Engineering (H. Madry).PART II: TISSUE ENGINEERING.6.Tissue Engineering: Basic Considerations (G. Vunjak-Novakovic).7.Tissue Engineering of Articular Cartilage (K. Masuda & R. Sah).8.Ligament Tissue Engineering (J. Chen, et al.).9.Cellular Photoencapsulation in Hydrogels (J. Elisseeff, et al.).10.Tissue Engineering Human Skeletal Muscle for Clinical Applications (J. Shansky, et al.).11.Engineered Heart Tissue (T. Eschenhagen & W. Zimmermann).12.Tissue-Engineered Blood Vessels (R. Klinger & L. Niklason).13.Tissue Engineering of Bone (S. Hofmann, et al.).14.Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering (P. Lelkes, et al.).15.Tissue Engineering of the Liver (G. Underhill, et al.).Suppliers List.Glossary.Index.