About the Book :
This book encompasses the most commonly used methods on basic and applied aspects of Nematology rather than compiling various techniques available in the literature. Emphasis has been given to make techniques comprehensible with sketches and photographs. Illustrated identification keys for common phytophagous nematode genera, species, and races of root knot, cyst and reniform nematodes, etc., have been included to meet the requirement of workers not well versed in nematode taxonomy. The book should prove to be indispensable for both under graduate and post graduate students, and researchers interested in working with the wonderful world of tiny creatures - the plant parasitic nematodes. Different exercises of the book have been written by the expert scientists having more than twenty five years of practical experience in the field of Nematology.
Exercise 1. Collection and preservation of soil and plant samples - Harish K. Bajaj Collection of soil samples Collection and preservation of plant parts Exercise 2. Isolation of nematodes from soil and plant parts and their estimation - Harish K. Bajaj Isolation and collection of nematodes from soil Isolation and collection of active nematodes and cysts Isolation and collection of sessile, slow moving or moulting nematodes Isolation and collection of nematodes from Plant Parts Isolation and collection of migratory endoparasitic nematodes Isolation and collection of sedentary endo- and semiendoparasitic forms Counting nematode population in suspension Exercise 3. Preparation of nematode slides for morphology and taxonomic studies - Harish K. Bajaj Preparation of temporary mounts Killing, fixing and preserving nematodes Preparation of permanent yoto mounts Preparation of perineal pattern, vulval cone and en face view Exercise 4. Measurements of nematodes - Harish K. Bajaj Measurements with an ocular micrometer Measurements with camera lucida Measuring nematodes and their body parts Exercise 5. Identification scheme for common genera of plant parasitic nematodes of India - Harish K. Bajaj Exercise 6. Identification of common cyst and root-knot nematode species of India - Harish K. Bajaj Identification of cyst nematodes Identification of Meloidogyne Species Exercise 7. Identification of host races of important nematode species of India - Harish K. Bajaj Identification of host races of Meloidogyne incognita, M. arenaria and M. javanica Identification of host races of Rotylenchulus reniformis Identification of host races of Heterodera cajani Identification of pathotypes of Heterodera avenae Identification of host races of Heterodera zeae Identification of host races of Globodera rostochiensis and G. pallida Exercise 8. Life cycle studies of nematodes - Harish K. Bajaj Embryonic development (Hanging drop method) Post embryonic development Variations with other major nematode groups Exercise 9. Histopathology of nematode infected roots - P.P.S. Baghel Exercise 10. Pathogenicity trials for plant parasitic nematodes - K.R. Dabur Exercise 11. Major nematode pests of important crops in India : Morphology, symptomatology and control - Harish K. Bajaj & R.S. Kanwar Anguina tritici Aphelenchoides species Ditylenchus angustus Heterodera and Globodera species Meloidogyne species Rotylenchulus reniformis Tylenchulus semipenetrans Radopholus similis Hirschmanniella species Pratylenchus species Exercise 12. Crop loss assessment due to nematodes - R.S. Kanwar & Harish K. Bajaj Estimation of avoidable losses in an infested Estimation of losses in a geographical area Exercise 13. In vitro screening of chemicals against nematodes - I.J. Paruthi Mortality Test Hatching Test Exercise 14. Testing of chemicals against plant parasitic nematodes by different methods in pots and field - R.K. Jain Evaluation of chemicals by seed soaking method for the control of root-knot nematode Efficacy of chemicals used as seed dressing method for the control of root-knot nematode Evaluation of chemicals for the control of root knot nematode through bare root-dip method Evaluation of granular nematicides for the control of root-knot nematode as soil application / nursery bed treatment Exercise 15. Testing phytotherapeutic substances for nematode control - R.S. Kanwar Evaluation of plant extracts for their toxicity against root-knot nematode Evaluation of efficacy of plant parts for root-knot nematode control Exercise 16. Screening of germpalsm for resistance against important phytoparasitic nematodes - R.K. Jain Meloidogyne species on vegetables and pulse crops Rotylenchulus reniformis on vegetable and pulse crops Anguina tritici on wheat Heterodera avenae on wheat and barley Heterodera cajani on pulse crops Tylenchulus semipenetrans on citrus Radopholus similis on coconut Ditylenchus angustus on rice Exercise 17. Field trials on biocontrol agents for the management of phytonematodes - R.K. Walia Paecilomyces lilacinus Pasteuria penetrans Exercise 18. Crop rotation trials for management of plant parasitic nematodes - R.S. Kanwar Exercise 19. In vitro culturing of plant parasitic and fungivorus nematodes - R. S. Kanwar In vitro culturing of plant parasitic nematodes Callus tissue technique Carrot disc technique Excised root technique In vitro culturing of fungal feeding nematodes Exercise 20. Isolation and culturing of entomopathogenic nematodes - Harish K. Bajaj Isolation and mass rearing of EPNs in vivo Collection of adults of EPNs and studies on their life cycles